Maryanne McClellan

EDUCATION

Auburn University, B.S., 1971
Colorado State University, M.S., 1975
Colorado State University, Ph. D., 1979
Oregon Regional Primate Center - NIH Postdoctoral Fellow, 1980-81

TEACHING

I teach an upper-division course in Cellular Biology, and I teach an advanced seminar course in Signal Transduction Pathways.

I also teach in two team-taught courses: Introductory Biology and Introduction to Experimental Biology

RESEARCH

I am broadly interested in vertebrate reproduction and specifically in female sex steroid action. Currently, my laboratory is focused on understanding estrogen receptor (ER) action. By binding to estrogen and other environmental compounds that mimic estrogen, ERs alter the expression of particular genes during development, reproduction and disease. ER status is an important therapeutic indicator in breast cancer, and presumably the disease state results in part from aberrant gene expression. The repertoire of genes that are direct targets of ERs is being unveiled by a number of laboratories and many of these estrogen-regulated genes are inhibited while others are activated, but the specific mechanism/s of repression are not understood. We employed a novel unbiased strategy to find genomic targets of ER association in cultured breast cancer cells. Using some of these newly discovered gene targets, we are studying how ERs act to inhibit their expression.

RECENT STUDENT THESES

The following is a list of senior theses completed by students in my lab during the past six years. To view some of these students' published abstracts go to: http://academic.reed.edu/biology/theses/index.html

2001	Jennifer Suzanne Carter Biology Quantitation of Estrogen Receptor Distribution in Feline Uterine and Oviductal Epithelial Cells Using a Green Fluorescent Protein Chimera Jennifer E. Robertson Biology Effects of Murine Cytomegalovirus (MCMC) Infection on the Interaction of MHC class I Molecules with the Peptide Loading Complex
2002	Jeffery Tyler Brown Biology Immunocytochemical Colocalization of Proteasomes and the Estrogen Receptor Katherine Corrine Johnson Biology Phosphorylative Effects upon Estrogen Receptor Function Larry Huang Biology 17ß Estradiol has Oposing Effects on p70 S6 Kinase Activity in Human Breast Cancer cells Richard Walter Martin Biology 17b-Estradiol Modulates the Profile of Extracellular Regulated Kinase (ERK) Activation Alone and in Combination with Insulin-like Growth Factor-I Paula Anne Newman Biology Tamoxifen May Act through a Calcineurin, but Not a p38, Mediated Pathway to Induce Apoptosis in MCF-7 Breast Cancer Cells Julia Staverosky BMB Herstatin Inhibition of Mitogen Activated Protein Kinase (MAPK) in Δ-Epidermal Growth Factor Receptor (Δ -EGFR) Driven Tumors is Insufficient to Block Tumor Formation
2003	Carrie Francis Dammon Biology Immunological Tolerance during Placental Reproduction: Overcoming the Juxtaposition of the Maternal Immune System and Fetal Antigens Karen Rae Foster Biology Possible Recruitment of Histone Deacetylase-1 to the Nuclear Matrix: Immunocytochemical Colocalization of Histone Deacetylase-1 and the Estrogen Receptor- Amy Elisabeth Hesse Biology Are Atrazine and Heat Making Frogs Croak?: An Investigation of the Endocrine Disruptor Paradigm in Amphibians Nancy Marie Van Prooyen Biochemistry and Molecular Biology Molecular Tango: Roles of Erk5 in Cell Differentiation and Death
2004	Molly Marie Ross O’Quinn Biology Deducing the JNK MAPK Pathway Using RNAi in Drosophila Cells Exposed to Oxidative Stress
2005	Jennifer Burian, Biology Retinoic Acid X Receptor α is an ER target gene in MCF-7 Breast Cancer Cells Ben Pedroja Biology My RIP, a Rab Effector, is a Novel Mammalian A-Kinase Anchoring Protein
2006	Christopher Thomas Boniface Biochemistry and Molecular Biology ß-Arrestin 1 is a Putative Target of 17-ß-Estradiol-Mediated Splice Site Regulation in MCF-7 Human Breast Cancer Cells Victoria Kathryn Jenkins Biology Effect of 17-ß Estradiol on Regulation of Retinoic Acid X Receptor a in Breast Cancer Cells Colin William Morrow Biology Towards an Understanding of the Intracellular Actions of Pre-B-Cell Colony Enhancing Factor Marc William Nolan Biology Finding Neverland: An Examination of Female Hormone Replacement Therapy Susanna Holley Wegner Biology Bisphenol A Enhances ER Recruitment to Estrogen-Responsive Genes in MCF-7 Breast Cancer Cells
2007	Leigh Ann Curran Biology The Effect of 17-ß Estradiol on FoxA1 Localization in MCF-7 Breast Cancer Cells Cooper Andrew French Biology GABAergic Signalling Regulates Growth Cone Motility in Newborn Neurons of the Dentate Gyrus Zachary Robert Lewis Biology Proliferation of Primordial Germ Cells and Sex Differentiation in Threespine Stickleback (Gasterosteus aculeatus)

The following students have completed independent research projects (separate from thesis research) in my laboratory during the past four years:

Kat DeLand, Oliver Schmachtenberg, John McDougall, Cindy Ellig, America Mahur, Michael Oh.

SELECTED PUBLICATIONS

McClellan, M.C., West, N.B., Tacha, D.E., and Brenner, R.M., 1984. Immunocytochemical localization of estrogen receptors in the macaque reproductive tract with monoclonal antiestrophilins. Endocrinology, 114:2002.

McClellan, M.C., West, N.B., and Brenner, R.M. 1986. Estrogen receptors in stromal and epithelial cells of the macaque endometrium during the luteal-follicular transition. Endocrinology, 119:2467.

Brenner, R.M., West, N.B. and McClellan, M.C. 1990. Estrogen and progestin receptors in the reproductive tract of male and female primates. Biol Reprod 42:1-10.

McClellan, M.C., Rankin, S., West, N.B. and Brenner, R.M. 1990 Estrogen receptors, progestin receptors and DNA synthesis in the macaque endometrium during the luteal-follicular transition. J. Steroid Biochem. Molec. Biol. 37:631-641.

Brenner, R.M., McClellan, M.C., West, N.B., Novy, M.J., Haluska, G.J., and Sternfeld, M.D. 1991. Estrogen and Progestin Receptors in the Macaque Endometrium. Annals New York Acad. Sci. 622:149.

Maintained by the Reed College Biology Department
Last Modified 8/10/99
Questions/Comments to Maryanne.McClellan@directory.reed.edu