Chemistry 401 :  Topics in Biochemistry - Fall 2007

Instructor:  Arthur Glasfeld , Chemistry 410, (503)517-7679

Meets Tues. 6:10-7:30 PM in ETC 205 (See Course Prospectus)

Remember: Final papers due at 5 PM on 12/13/07


Nov. 27th: Metal Chaperones - class meeting at Arthur's house

Rae et al. (1999) Undetectable Intracellular Free Copper Science 284, 805-808.

Torres et al. (2001) Copper Stabilizes a Heterodimer of the yCCS Metallochaperone. J. Biol. Chem. 276, 38410-38416.

Nov. 20th: Superoxide Reductase.

Molina-Heredia et al. (2006) Detoxification of superoxide without H2O2: Antioxidant activity of superoxide reductase complexed with ferrocyanide PNAS 103, 14750-14755.

Katona et al. (2007) Raman-Assisted Crystallography Reveals End-On Peroxide Intermediates in a Nonheme Iron Enzyme. Science 316, 449-453.

Nov. 13th: RNA Enzymes.

Stahley and Strobel (2005) Structural Evidence for a Two-Metal-Ion Mechanism of Group I Intron Splicing. Science 309, 1587-1590.

Forconi et al. (2007) Modulation of individual steps in group I intron catalysis by a peripheral metal ion. RNA 13, 1656-1667.

Nov. 6th: Restriction Enzymes. Pymol files for CreI and EcorRV are available.

Baldwin et al. (2001) DNA cleavage by the EcoRV restriction endonuclease: roles of metal ions in specificity and catalysis. J. Mol Biol. 288, 87-103.

Chevalier et al. (2004) Metal-Dependent DNA Cleavage Mechanism of the I-CreI LAGLIDADG Homing Endonuclease. Biochem. 43, 14015-14026.

Oct. 23rd: Metalloregulatory proteins. Note that both these papers come from Science, which places experimental detail in captions and references.

Outten, C. E., and O'Halloran, T. V. (2001) Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis. Science 292, 2488-2492.

Changela et al.(2003) Molecular Basis of Metal-Ion Selectivity and Zeptomolar Sensitivity by CueR Science 301, 1383-1387.

Oct. 9th: A pymol file with the HMG-cisplatinated DNA structure

Hartwig & Lippard(1992) DNA Binding Properties of cis-sort of platin, a Metabolite of an Orally Active Platinum Anticancer Drug. J. Am. Chem. Soc. 114, 5646-5654.

Jamieson et al.(1999) Structural and Kinetic Studies of a Cisplatin-modified DNA Icosamer Binding to HMG1 Domain B J. Biol Chem. 274, 12346-12354.

Sept. 25th: A pymol file with the channel structure

Heginbotham et al.(1998) Functional reconstitution of a Prokaryotic K+ Channel, J. Gen. Phys. 111, 741

Morais-Cabral et al.(2001) Energetic optimization of ion conduction rate by the K+ selectivity filter. Nature 414, 37.

Sept. 18th:

Uversky et al. (2001) Metal-triggered Structural Transformations, Aggregation, and Fibrillation of Human alpha-Synuclein. J. Biol. Chem. 276, 44284-44296.

Sept. 11th:

Payne et al. (1999) Lead Fingers:  Pb2+ Binding to Structural Zinc-Binding Domains Determined Directly by Monitoring Lead-Thiolate Charge-Transfer Bands.  J. Am. Chem. Soc.121, 6850-6855.

Gehring et al. (2005) Spectroscopic and Functional Determination of the Interaction of Pb2+with GATA Proteins.  J. Am. Chem. Soc. 127, 3751-3759.

Sept. 4th:

Martinez et al. (2003) Structure and membrane affinity of a suite of amphiphilic siderophores produced by a marine bacterium. Proc. Nat. Acad. Sci. USA 100, 3754.

Küpper et al. (2006) Photoreactivity of Iron(III)-Aerobactin: Photoproduct structure and Iron (III) Coordination. Inorg. Chem. 45, 6028.

Aug. 28th:

Course introduction. Some notes on metals and biochemistry.